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recombinant human asprosin (Revvity)

Name: recombinant human asprosin
Brand: Revvity
Rating: 96 (1308 reviews)

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Revvity recombinant human asprosin
In vitro overexpression of <t>asprosin</t> Asprosin levels measured using ELISA in HEK293T cell conditioned media collected 72 h post transfection with 10 μg of pcDNA3.1 (empty vector control) or pcDNA3.1-IL2sp-6his-Asprosin. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

In vitro overexpression of <t>asprosin</t> Asprosin levels measured using ELISA in HEK293T cell conditioned media collected 72 h post transfection with 10 μg of pcDNA3.1 (empty vector control) or pcDNA3.1-IL2sp-6his-Asprosin. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Recombinant Human Asprosin, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1308 article reviews

recombinant human asprosin - by Bioz Stars, 2026-02

96/100 stars

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1) Product Images from "Overexpression and ELISA-based detection of asprosin in cultured cells and mice"

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

Journal: STAR Protocols

doi: 10.1016/j.xpro.2022.101762

In vitro overexpression of asprosin Asprosin levels measured using ELISA in HEK293T cell conditioned media collected 72 h post transfection with 10 μg of pcDNA3.1 (empty vector control) or pcDNA3.1-IL2sp-6his-Asprosin. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Figure Legend Snippet: In vitro overexpression of asprosin Asprosin levels measured using ELISA in HEK293T cell conditioned media collected 72 h post transfection with 10 μg of pcDNA3.1 (empty vector control) or pcDNA3.1-IL2sp-6his-Asprosin. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Techniques Used: In Vitro, Over Expression, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation

Viral overexpression of human asprosin results in increased appetite and body weight gain in lean mice (A and B) cumulative food intake and body weight change were measured 15- and 18-days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with Ad5-empty or Ad5-h FBN1 (3.6 × 10 9 pfu/mouse) viral vector. (C and D) Cumulative food intake and body weight change were measured 47 days and 60 days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with AAV8-empty or AAV8-IL2sp-6His-Asprosin (1 × 10 12 GC/mouse) viral vector. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Figure Legend Snippet: Viral overexpression of human asprosin results in increased appetite and body weight gain in lean mice (A and B) cumulative food intake and body weight change were measured 15- and 18-days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with Ad5-empty or Ad5-h FBN1 (3.6 × 10 9 pfu/mouse) viral vector. (C and D) Cumulative food intake and body weight change were measured 47 days and 60 days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with AAV8-empty or AAV8-IL2sp-6His-Asprosin (1 × 10 12 GC/mouse) viral vector. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Techniques Used: Over Expression, Injection, Plasmid Preparation

In vivo overexpression of Asprosin using adenoviral vector (Ad5) (A and B) Human asprosin levels detected in plasma of Ad5-empty (control) and Ad5-h FBN1 injected C57BL/6J male mice, 12 days (A) and 25 days (B) after viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in Ad5-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Figure Legend Snippet: In vivo overexpression of Asprosin using adenoviral vector (Ad5) (A and B) Human asprosin levels detected in plasma of Ad5-empty (control) and Ad5-h FBN1 injected C57BL/6J male mice, 12 days (A) and 25 days (B) after viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in Ad5-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Techniques Used: In Vivo, Over Expression, Plasmid Preparation, Injection, Transduction

In vivo overexpression of Asprosin using adeno-associated viral vector 8 (A and B) Human asprosin levels detected in plasma of AAV8-empty (control) and AAV8-IL2sp-6His-Asprosin injected C57BL/6J male mice, 25 days (A) and 55 days (B) after adeno-associated viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in AAV8-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Figure Legend Snippet: In vivo overexpression of Asprosin using adeno-associated viral vector 8 (A and B) Human asprosin levels detected in plasma of AAV8-empty (control) and AAV8-IL2sp-6His-Asprosin injected C57BL/6J male mice, 25 days (A) and 55 days (B) after adeno-associated viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in AAV8-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Techniques Used: In Vivo, Over Expression, Plasmid Preparation, Injection, Transduction

Figure Legend Snippet:

Techniques Used: Concentration Assay, Recombinant, Protease Inhibitor, Transfection, Blocking Assay, Cell Culture, Plasmid Preparation

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Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: In vitro overexpression of asprosin Asprosin levels measured using ELISA in HEK293T cell conditioned media collected 72 h post transfection with 10 μg of pcDNA3.1 (empty vector control) or pcDNA3.1-IL2sp-6his-Asprosin. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: In Vitro, Over Expression, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: Viral overexpression of human asprosin results in increased appetite and body weight gain in lean mice (A and B) cumulative food intake and body weight change were measured 15- and 18-days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with Ad5-empty or Ad5-h FBN1 (3.6 × 10 9 pfu/mouse) viral vector. (C and D) Cumulative food intake and body weight change were measured 47 days and 60 days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with AAV8-empty or AAV8-IL2sp-6His-Asprosin (1 × 10 12 GC/mouse) viral vector. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: Over Expression, Injection, Plasmid Preparation

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: In vivo overexpression of Asprosin using adenoviral vector (Ad5) (A and B) Human asprosin levels detected in plasma of Ad5-empty (control) and Ad5-h FBN1 injected C57BL/6J male mice, 12 days (A) and 25 days (B) after viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in Ad5-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: In Vivo, Over Expression, Plasmid Preparation, Injection, Transduction

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: In vivo overexpression of Asprosin using adeno-associated viral vector 8 (A and B) Human asprosin levels detected in plasma of AAV8-empty (control) and AAV8-IL2sp-6His-Asprosin injected C57BL/6J male mice, 25 days (A) and 55 days (B) after adeno-associated viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in AAV8-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: In Vivo, Over Expression, Plasmid Preparation, Injection, Transduction

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet:

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: Concentration Assay, Recombinant, Protease Inhibitor, Transfection, Blocking Assay, Cell Culture, Plasmid Preparation

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